目的 研究9个不同引种柴胡地上部不同器官、不同生长期总黄酮含量变化规律,探讨最佳采收期及最优品种。方法 采用微波辅助优化提取条件,紫外分光光度法测定黄酮含量。结果 最佳提取条件:乙醇浓度80%,每次微波时间40 s,微波10次;不同生长期不同器官总黄酮含量差异较大,呈上下波动趋势;花中含量最高,在4.65%~16.75%内,平均为7.59%,营养生长期总黄酮含量:叶>茎,花期含量:花>叶>茎;果期:甘肃陇西、辽宁沈阳、山东菏泽和河北安国柴胡:果>叶>茎,其他柴胡:叶>果>茎;9个引种柴胡在花期和果期总黄酮含量较高,以甘肃陇西柴胡(初花期)含量最高,可达27.41%。结论 柴胡地上部分含有较高黄酮,建议在花期或果期进行采收,对其进行综合开发利用。
Abstract
OBJECTIVE To study variation of total flavonoids from nine different Bupleurum chinense introduced of different ground parts of different organs in different growth period, and explore the best harvest period and the optimal varieties. METHODS The extraction conditions were optimized by microwave assisted method,UV-visible spectropholometry was used to determine the contents of total flavonoids. RESULTS The optimum extraction conditions:the concentration of ethanol was 80%, each microwave time 40 s, microwave 10 times; the content of total flavonoids was difference in different growth period, fluctuated trend. The flower was the highest, with an average of 7.59% in the range of 4.65%-16.75%,the content of total flavonoids in nutrition growth phase:leaf>stem, in flowering stage:flower>leaf>stem; in fruiting stage:the content of Gansu Longxi, Liaoning Shenyang, Shandong Heze and Hebei Anguo Bupleurum:fruit >leaf> stem, other Bupleurum:leaf >stem>fruit;The higher the content of total flavonoids was in the flowering and fruiting of nine different Bupleurum chinense introduced, Gansu Longxi Bupleurum (flowering stage) was the highest, up to 27.41%. CONCLUSION It is suggested that harvested in the flowering or fruiting stage,and carried on the comprehensive development and utilization.
关键词
柴胡 /
总黄酮 /
紫外分光光度法 /
花
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Key words
Bupleurum chinense /
total flavonoids /
UV-visible spectropholometry /
flower
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中图分类号：
S322.1
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参考文献
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基金
国家科技部子课题项目(2011BAI07B05-4);唐山市科技重大项目(12130203A);唐山师范学院科技平台建设项目(11PT01-2)
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